A basic thiazine dye that is related to methylene blue and is used as a biological stain. Based on the chemistry associated with this set of interrelated compounds cf. Wrights stain colors red blood cells bluepurple, while turning white blood cells and platelets other colors. Toluidine blue stains the nucleus and cytoplasm blue, but mast cells purple. Toluidine blue and toluidine blue nanogold mixtures were incorporated into polyurethane and silicone polymers by a swellencapsulationshrink method using acetonewater mixtures.
A positive toluidine blue test causes the solution to turn from blue to pink. Section 1 chemical product and company identification. Over the last couple weeks ive received several questions related to using toluidine blue dye in sexual assault medicalforensic. Toluidine blue is a cationic metachromatic dye that may selectively bind to free anionic groups such as sulfate, phosphate, and carboxylate radicals of large molecules 5, 34.
Epub is an open e book standard recommended by the international digital publishing forum which is designed for reflowable. What is best preparation for toluidine blue reagent for. Some protocol mention dissolving toluidine blue in distilled water, some in ethanol and some in acetate buffer. Although toluidine blue o is used routinely as a stain in animal cytol ogy, and. Tolonium chloride, basic blue 17 company name ted pella, inc. Toluidine blue tblue stain has been shown to be selective for staining of premalignant and malignant mucosal lesions fig. Toluidine blue tb dye molecules are intensively utilized for largearea photophysics applications such as carcinoma detection.
Ricca chemical 865432 toluidine blue aqueous solution, 1% wv, 1l glass amber container currently unavailable. The solutions were then slowly cooled and a renaturation curve was obtained. Toluidine blue, 1% aqueous safety data sheet according to federal register vol. Antimicrobial activity of methylene blue and toluidine blue o. Hematoxylin and eosin produce a permanent stain, so slides made using this combination may be kept for later examination. Toluidine blue dye tbd application used for mfsaeec 1 toluidine blue dye tbd application will be done prior to any digital, speculum examination, or vaginal instrumentation i. Material safety data sheet toluidine blue, 1% aqueous.
Toluidine blue test definition of toluidine blue test by. Division of radiooncology, deutsches krebsforschungszentrum, 69120 heidelberg, germany subsequent to immunohistological reaction, counterstaining of tissue sections with the thiazin dye toluidine blue toluidine blue o is an alternative to other dyes e. Toluidine blue staining staining protocol sample type required. Toluidine blue definition of toluidine blue by medical. Nist chemistry webbook the national institute of standards and technology nist uses its best efforts to deliver a high quality copy of the database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. Toluidine blue dye leave a comment over the last couple weeks ive received several questions related to using toluidine blue dye in sexual assault medicalforensic exams, so i figured it was time to put together a clinical guide on the topic. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage. Wrights stain colors red blood cells blue purple, while turning white blood cells and platelets other colors. Supramolecular organization of toluidine blue dye in solid. It is a blue nuclear counterstain that can be used to demonstrate nissl substance and is also useful for staining mast cell granules, both in metachromatic and orthochromatic techniques.
Toluidine blue o is a blue cationic basic dye used in histology to test for lignin and for staining semithin 0. Tolonium chloride is an organic chloride salt having 3amino7dimethylamino2methylphenothiazin5ium as the counterion. To do this staining, after air drying of smears, they were fixed in fresh 96% ethanolacetone 1. Toluidine blue, also known as tbo or tolonium chloride inn is a blue cationic basic dye. Search results for toluidine blue o at sigmaaldrich. Toluidine blue and toluidine bluenanogold mixtures were incorporated into polyurethane and silicone polymers by a swellencapsulationshrink method using acetonewater mixtures. The metabolism of 14c o toluidine in both bivalve species resulted in the formation of 2nitrosotoluene, nmethylo toluidine and nformylo toluidine. The effect of toluidine blue on the first and second melting curves of rrna from the mouse liver. Toluidine definition is any of three isomeric amino derivatives of toluene c7h9n that are analogous to aniline and are used as dye intermediates. Hmis 0 to 4 this material is not considered hazardous. Toluidine definition of toluidine by merriamwebster. Uvvisible absorption spectra confirmed that the dye was surface. Chondrogenicinduced pellets from all investigated cell sources showed intense purple metachromasia in toluidine blue staining, indicating a high content of sulfated proteoglycans figure 7ae.
Toluidine blue color perception in identification of oral. Toluidine blue o, 1% aqueous, laboratory grade, 100. Department of physics, tamilnadu college of engineering, karumatham patti, coimbatore641 659, india. Chondrogenic differentiation was assessed histologically by demonstrating the presence of cartilagerelated matrix components in the specimens. Toluidine blue stock solution 5 ml 1% sodium chloride, ph 2. Although he was working on the synthesis of quinine, perkin instead produced a blue substance with good dyeing. Chondrogenic differentiation, toluidine blue staining. Toluidine blue dye tbd application used for mfsaeec.
A similar test can be performed with phloroglucinolhcl solution, which turns red other histological uses. Sigmaaldrich offers a number of toluidine blue o products. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells. Toluidine blue, 1% biological stain lab chemical 15ml. Mar 24, 2019 toluidine blue stains the nucleus and cytoplasm blue, but mast cells purple. The metabolism of 14c otoluidine in both bivalve species resulted in the formation of 2. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in dna and rna. Toluidine blue solution is used in testing for lignin, a complex organic molecule that bonds to cellulose fibres and strengthens and hardens the cell walls in plants. Toluidine blue stains esophageal malignancies and dysplasia, although lugols iodine solution has supplanted it to a great extent. Jan 28, 2014 toluidine blue tb is a polychromatic dye which can absorb different colours depending on how it binds chemically with various tissue components.
Small amounts of nhydroxyo toluidine were detected in experiments with mytilus. Composition information on ingredients toluidine blue o 92319, 0. Sigmaaldrich, 198161, toluidine blue o certified by the biological stain commission, 92319, 25g. Structure, properties, spectra, suppliers and links for. Toluidine blue staining is useful for the enumeration of basophils and mast cells. The study of toluidine blueribosomal rna complexes. Pdf toluidine blue is a basic thiazine metachromatic dye with high affinity for. This material is not considered hazardous by the osha hazard communication standard 29 cfr 1910. As it is absorbed by nucleic acids, toluidine blue stains malignant or dysplastic lesions with elevated mitotic activity. Tolonium chloride an overview sciencedirect topics. Nist chemistry webbook the national institute of standards and technology nist uses its best efforts to deliver a high quality copy of the database. When evaluated as a part of the clinical examination, toluidine blue staining may provide additional information. Methylene blue or toluidine blue o were covalently bound to an activated silicone polymer by means of an amide condensation reaction. Toluidine blue o, 1% aqueous, laboratory grade, 100 ml.
This article provides an overview on chemistry, technique, and the various applications of toluidine blue. The biotranformation of o toluidine was determined by the extraction and analysis of eliminated metabolites. The biotranformation of otoluidine was determined by the extraction and analysis of eliminated metabolites. Toluidine blue is a biological stain for staining nuclear material during mitosis.
Division of radiooncology, deutsches krebsforschungszentrum, 69120 heidelberg, germany subsequent to immunohistological reaction. Except where otherwise noted, data are given for materials in their standard state at 25 c 77 f, 100 kpa. The surface and mechanical properties of the polymers were changed by the swellshrink process especially the youngs modulus, but not by the introduction of toluidine. It is widely used as a vital stain to distinguish the viable cells from the nonviable cells. It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology.
Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. In vivo, toluidine blue stains deoxyribonucleic acid andor. Toluidine blue, 1 percent wv aqueous solution is a blue cationic basic dye used in histology to test for lignin and for staining semithin 0. Chondrogenicinduced pellets from all investigated cell. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for acetoorcein stain. Toluidine blue stain an overview sciencedirect topics. Basic blue 17 chemical properties,uses, production. A similar test can be performed with phloroglucinolhcl solution, which turns red. Carolina offers the highest quality kits for a handson approach within ap chemistry classrooms. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. Himedia grm25g toluidine blue o, practical grade, 25 g. This material is not considered hazardous by the osha hazard communication standard 29 cfr. In the present investigation the absorption and emission spectral studies of toluidine blue o with sds to understand the formation of photoactive monomer dyes and. A toxic metachromatic thiazine dye used to stain thick sections for electron microscopy, which may cause haematuria due to haemorrhagic cystitis and methaemoglobulinuria.